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Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
www.genome.ou.edu
Design Oligonunucleotide Primers
Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.
sciborg.uwaterloo.ca
FISH Guide and Troubleshooting
Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.
info.med.yale.edu
List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
www.pcrlinks.com
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
twod.med.harvard.edu
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
www.hifidna.com
Presentations from the University of California at Berkley on PCR, both current research reports and reviews.
sunsite.berkeley.edu
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
www.cas.psu.edu
An introduction by Connie Veilleux from the US National Health Museum website.
www.accessexcellence.org
Polymerase Chain Reaction (PCR)
A graphic description of the principle of PCR from the US National Health Museum web site.
www.accessexcellence.org
Rep-PCR Genomic Fingerprinting
Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
www.msu.edu
A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.
info.med.yale.edu
Popular description of the PCR technique by John C Brown, University of Kansas 1995.
people.ku.edu
Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.
www.accessexcellence.com
Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
www.invent.org