CLED agar

From Wikipedia, the free encyclopedia

CLED agar (Cysteine Lactose Electrolyte Deficient medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary organisms. Being electrolyte deficient, it prevents the swarming of Proteus species. Lactose fermenters produce yellow colonies on CLED agar; non-lactose fermenters appear blue. It has a pH of approximately 7.3. CLED agar contains:

Peptone 4g/l
'Lab Lemco' powder 3g/l
Tryptone 4g/l
Lactose 10g/l
L-Cysteine 128mg/l
Bromothymol blue 20mg/l
Agar No. 1 15g/l
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