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Microtiter plate
From Wikipedia, the free encyclopedia
A Microtiter plate or microplate is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. It typically has 6, 24, 96, 384 or even 1536 sample wells arranged in a 2:3 rectangular matrix. Some microplates have even been manufactured with 3456 or even 9600 wells. Each well of a microplate typically holds somewhere between a few to a few hundred microliters of liquid.
The earliest microplate was created in 1951 by a Hungarian, Dr. G. Takatsky, who machined 6 rows of 12 "wells" in Lucite. However, common usage of the microplate began in the late 1950s when John Liner in USA had introduced a molded version. By 1990 there were more than 15 companies producing a wide range of microplates with different features. It was estimated that 125 million microplates were used in 2000 alone.
In 1996, the Society for Biomolecular Screening (SBS) began an initiative to create a standard definition of a microtiter plate. A series of standards was completed in 2003 and published by the American National Standards Institute (ANSI) on behalf of the SBS. The standards governs various characteristics of a microtiter plate including well dimensions (e.g. diameter, spacing and depth) as well as plate properties (e.g. dimensions and rigidity).
A number of companies have developed robots to specifically handle SBS microplates. These robots may be liquid handlers which aspirate or dispense liquid samples from and to these plates, or "plate movers" which transport them between instruments.
Instrument companies have designed plate readers which can detect specific biological, chemical or physical events in samples stored in these plates.
- Microplate History 2nd Edition May 1999 by Roy Mann
- Society for Biomolecular Screening
- A sample image of some 96-well microtiter plates
- American National Standards Institute
- Glass Microtiter Plates
Agar plate • Aspirator • Autoclave • Bunsen burner • Calorimeter • Colony counter • Colorimeter • Centrifuge • Fume hood • Incubator • Laminar flow cabinet • Magnetic stirrer • Microscope • Microtiter plate • Plate reader • Spectrophotometer • Stir bar • Thermometer • Vortex mixer • Static mixer
Laboratory glassware
Beaker • Boiling tube • Büchner funnel • Burette • Condenser • Conical measure • Crucible • Cuvette • Laboratory flasks (Erlenmeyer flask, Round-bottom flask, Florence flask, Volumetric flask, Büchner flask, Retort) • Gas syringe • Graduated cylinder • Pipette • Petri dish • Separating funnel • Soxhlet extractor • Test tube • Thistle tube • Watch glass
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