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Queen Bee is the name of four different DC Comics supervillain.

The leader of the hiveworld Korll, Zazzala lives only for the interstellar expansion of her species. Zazzala first appeared in Justice League of America #23 (November 1963). She clashed frequently with the original Justice League during the sixties, but largely disappeared for several decades.

Zazzala reappeared in JLA volume 3, #34 (October 1999), when Lex Luthor contacted her to join his Injustice Gang. She agreed, freeing The General from his asteroid prison in exchange for a percentage of Earth's population to become her drones. Her Bee-Troopers headed toward Earth and after hypnotizing the citizens of New York, she forced them to plant an egg matrix which would give her command of the population. She also attempted to use her hypno-pollen to take control of Green Lantern and Steel, but both heroes were able to fight it off; Huntress encouraged Green Lantern to resist the pollen, and Steel's suit protected him from the pollen's effects. Using the Queen Bee's inability to see the colour red, while Wonder Woman and Big Barda kept the Queen's forces occupied, Steel and Plastic Man sneaked up to the Queen and banished her and her army back to Korll via a Boom Tube.

Most recently, Zazzala and her drones have joined Lex Luthor's most recent venture, the new Secret Society of Super Villains and also became leader of the H.I.V.E.. Zazzala has insect-like superhuman strength and speed, can fire venomous stinger darts, or release mind-confusing "pollen" powder. She and her drones are unable to perceive the color red.

An unrelated Queen Bee was introduced in Justice League International #16 (August 1988). She was an unpowered, human femme fatale, who gained control of the terrorist nation of Bialya by forging an alliance with its' former ruler Colonel Rumaan Harjavti then assassinating him. She solidified her power by brainwashing the Global Guardians into serving her. She has clashed several times with Justice League International and Justice League Europe.

Justice League Europe found out that the Queen Bee was behind their recent troubles, and that she had a Dominator named Doctor working for her. They invaded her palace and came to an agreement; she would cease hostilities against them if they kept quiet about the unethical things she was doing as Queen. They also demanded she sever relations with the Doctor. After they left, she killed the Doctor.

The Queen Bee was eventually defeated by the JLE, the Guardians and Rumaan Harjavti's brother Sumaan, during the events of the JLA/JLE crossover Breakdowns (1991).

Queen Bee III appeared in the 2000 Creature Commandos series. On the otherdimensional world of Terra Arcana Tazzala, sister of Zazzala, the original Queen Bee, joined Simon Magus' Terra Arcana Army with the ultimate goal of conquering Earth. The U.S. Army faction known as the Creature Commandos stopped the invasion plans. Tazzala herself was killed by Simon Magus.

The sister of the second Queen Bee, Beatriz was introduced in JLA: Incarnations #6 (December 2001, but set around 1996) as the new ruler of Bialya. She using humans forged with machinery and passed off as the robot Extremists to police her country. Her fate since Captain Atom's Extreme Justice team brought an end to these activities is unknown, but she was presumably toppled from power as she no longer rules Bialya.

• In Justice League Unlimited, Queen Bee is seen as a member of Gorilla Grodd's Legion of Doom in the third season premiere, but she does not make a full appearance in the animated series. However, in the episode "Far From Home," Supergirl fights robotic duplicates of Zazzala and Doctor Cyber for training purposes. The "real" animated Queen Bee has thus far only appeared in a major role in the Justice League Unlimited comic (issue #29), in which she is the primary villain. She is defeated by the combined efforts of Animal Man and B'wana Beast
• She is one of the major bosses in the Justice League Heroes game for Playstation 2 and Playstation Portable. She was voiced by Abby Craden.
• In the Smallville episode "Drone", a girl named Sasha Woodman has the ability to control swarms of bees, though she is not referred to directly as Queen Bee.