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Ryan Wolfe is a fictional character in the show CSI: Miami, portrayed by American actor Jonathan Togo.

Ryan Wolfe, a former patrol officer, is hired after Tim Speedle is killed on the job. He has a strong background in science, majoring in chemistry at a Boston college and now pursuing a master's degree in genetics. His studies take a back seat to his job; he graduated top of his class at the police academy, well on his way to a promotion.

He catches the attention of Horatio Caine at the scene of a bus crash. There, Wolfe shows great attention to detail and to the care of his sidearm, reflecting the fixation on minute details emerging from his obsessive-compulsive disorder. Wolfe's eye for detail wins Caine's respect, since poor gun maintenance led to the misfire at a critical moment that contributed to Speedle's death (episode 301, "Lost Son").

When he first joins the lab, Wolfe has a difficult time with his co-workers. Eric Delko, who had been close to Speedle, is still angry over his friend's murder, and resents Wolfe as an interloper. Calleigh Duquesne, on the other hand, is concerned because Wolfe's first case is a possible vehicular homicide involving her alcoholic father, lawyer Kenwall "Duke" Duquesne (episode 303, "Under the Influence"). Over time, however, Wolfe has proved his worth to the crime lab, being more concerned with solving cases than with his personal advancement.

In the episode 408 ("Nailed"), Wolfe is shot through his eye with a nail from a nail gun at a crime scene, leaving him with vision problems. He misses a key piece of evidence during episode 412 ("The Score"), and approaches coroner Alexx Woods for help. She had previously prescribed him an antibiotic (episode 410, "Shattered"), and is concerned that his eye troubles are more serious than previously thought.

During fourth season, Ryan works to uncover the identity of the mole in the lab, after reporter Erika Sykes reveales the existence of an informer (special scene at cbs.com [extended scene] following episode 409, "Hellraisers"). In the episode 425 ("One Of Our Own"), while the FBI is investigating the lab following an anonymous tip about supposed corruption, an agent brings up the possibility that Wolfe has keratitis, a condition that causes impaired vision, and implies that since Ryan was supposedly the last to handle the missing money from evidence, he stole it for himself. After this interrogation, Ryan goes to lab technician Dan Cooper and slams him against a wall, accusing him of being the mole in the lab, as Cooper is the only one aside from Alexx Woods who knew of his vision troubles. Cooper pleads his innocence, saying he merely let the information slip after one too many beers. Afterwards, Wolfe helps prove that the lab is not responsible for several thousand dollars in money missing from evidence, and that the last person to handle it had not been from their lab. There is powder from a latex glove on the re-taped evidence envelope, but the Miami-Dade Crime Lab doesn't use that kind of glove because it interferes with evidence. This leads Calleigh to Peter Elliot, of the Treasury Department, who in turn mournfully exposes the true culprit: Elliot's fiancee, State Attorney Monica West (episode 425, "One of Our Own").

Wolfe does occasionally let his emotions interfere with his job; in one episode, he stops a family from getting evicted from their home, and shoves the officer forcing the evictions. The officer files a complaint against Wolfe, which could have damaged his career. Caine convinces the officer to drop the complaint, but warns Wolfe that he might not be able to protect him next time (episode 505, "Death Eminent"). When colleague and friend Eric Delko is critically injured in the line of duty, Ryan and Calleigh Duquesne go to the hospital to give him blood, though they learn that it is too late for transfusions due to the sheer amount of blood loss. Ryan and the others then take turns at his bedside before going out to catch the perpetrators. Wolfe becomes angry to near-violence when they discover Eric's shooter, but manages to pull himself together (episode 515, "Man Down").

After Delko returns to work, he experiences difficulty in the performance of his duties, such as when he unknowingly doubles up evidence markers at a crime scene. Ryan notices this and sympathizes with him, recalling his own vision issues and frustration following his eye injury of the previous season (episode 518, "Triple Threat").

• CSI: Miami
• Jonathan Togo